| 1607-1609 | Gabriel Archer, Recorder |
| 1610-1611 | William Strachey |
| 1611-1614 | Ralph Hamor, Jr |
| 1614-1619 | John Rolfe |
| 1619-1621 | John Pory |
| 1621-1623 | Christopher Davison |
| 1625-1635 | William Claiborne |
| 1635-1649 | Richard Kemp |
| 1649-1652 | Richard Lee |
| 1652-1660 | William Claiborne |
| 1661-1678 | Thomas Ludwell |
| 1678 | Philip Ludwell |
| 1678-1679 | Daniel Parke |
| 1679-1689 | Nicholas Spencer |
| 1689-1692 | William Cole |
| 1692-1693 | Christopher Robinson |
| 1693-1701 | Ralph Wormeley |
| 1702-1712 | Edmund Jennings |
| 1712-1720 | William Cocke |
| 1720-1722 | Edmund Jennings |
| 1712-1743 | John Carter |
| 1743-1788 | Thomas Nelson |
| 1788-1800 | John Harvie |
| 1801-1811 | Daniel L. Hylton |
| 1811-1820 | William Robertson |
| 1820-1821 | John Burfoot |
| 1821-1852 | William H. Richardson |
| 1853-1865 | George W. Munford |
| 1865-1867 | Charles H. Lewis |
| 1867-1869 | John M. Herndon |
| 1869-1870 | Brevet Col. Garrick Mallery (appointed under Special Orders No. 68, Hdqrts., First Military District) |
| 1870-1879 | James McDonald |
| 1880-1881 | Thomas T. Flournoy* |
| 1882-1883 | William C. Elam |
| 1884-1893 | Henry W. Flournoy |
| 1894-1900 | Joseph T. Lawless |
| 1901-1910 | David Q. Eggleston |
| 1910-1926 | B. O. Jones |
| 1927-1929 | Martin A. Hutchinson |
| 1930-1937 | Peter H. Saunders |
| 1938-1941 | Raymond L. Jackson |
| 1942-1944 | Ralph E. Wilkins |
| 1945-1946 | Thelma Y. Gordon, acting |
| 1946-1948 | Jesse W. Dillon |
| 1948 | M. W. Armistead |
| 1948-1952 | Thelma Y. Gordon |
| 1952-1970 | Martha Bell Conway |
| 1970-1974 | Cynthia Newman |
| 1974-1978 | Patricia Perkinson |
| 1978 | Stanford E. Parris |
| 1978-1981 | Frederick T. Gray, Jr. |
| 1981-1982 | Marilyn Lussen, acting |
| 1982-1985 | Laurie Naismith |
| 1985-1986 | H. Benson Dendy, III |
| 1986-1990 | Sandra D. Bowen |
| 1990-1993 | Pamela M. Womack |
| 1993 | Scott Bates |
| 1993-1994 | Penelope Anderson, acting |
| 1994-1998 | Betsy Davis Beamer |
| 1998-2002 | Anne P. Petera |
| 2002-2006 | Anita A. Rimler |
| 2006 | Daniel G. LeBlanc |
| 2006-2010 | Katherine K. Hanley |
| 2010-2014 | Janet V. Kelly |
| 2014-2016 | Levar M. Stoney |
| 2016-2022 | Kelly T. Thomasson |
| 2022-2023 | Kay Coles James |
| 2023-Present | Kelly Gee |
*From 1801-1830 this official was designated only as “Clerk of the Council of State” or “Clerk of the Privy Council”; later the phrase “Keeper of the Public Seal” was added.